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Detection of Mucosal and Serum Antibodies Specific for the Capsular Polysaccharide of Haemophilus influenzae Type b by Enzyme‐Linked Immunosorbent Assay
Author(s) -
Harada Teruhiko,
Sakakura Yasuo,
Shimura Keishiro
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00863.x
Subject(s) - antibody , haemophilus influenzae , microbiology and biotechnology , biology , polysaccharide , bacterial capsule , enzyme , virology , immunology , biochemistry , virulence , gene , antibiotics
A sensitive enzyme‐linked immunosorbent assay (ELISA) with rough‐surfaced glass beads used as the solid phase was developed for detection of IgG, IgM, and IgA antibodies to Haemophilus influenzae type b capsular polysaccharide (HITB‐CP). A successful method for indirect coating of glass bead surfaces with HITB‐CP, and parameters affecting the specificity and sensitivity of the assay are described. This ELISA system proved to be 100 times as sensitive as the standard indirect fluorescent‐antibody assay. The assay was applied to the measurement of antibodies to HITB‐CP in serum and nasal secretions and proved to be a useful tool in the evaluation of immunological response to HITB infection.