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Nucleotide Sequence of the Replication Region of Plasmid R401 and Its Incompatibility Function
Author(s) -
Tabughi Akira
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00839.x
Subject(s) - replicon , plasmid , biology , genetics , nucleotide , open reading frame , nucleic acid sequence , direct repeat , coding region , inverted repeat , microbiology and biotechnology , homology (biology) , peptide sequence , dna , amino acid , gene , base sequence , genome
The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini‐Rtsl previously reported. The mini‐R401 was found to be composed of two clusters of direct repeated sequences flanking a large open reading frame that could encode a 33,000 Mr protein (RepA protein) consisting of 288 amino acids. This structure of mini‐R401 is quite similar to that of mini‐Rtsl. Furthermore, the nucleotide sequence of mini‐R401 is identical to that of mini‐Rtsl except for eleven nucleotides; three are located near the carboxyl terminus portion of the RepA coding region ( repA ) and four are in the repeated sequences ( incI ) located downstream from repA . Incompatibility study showed that mini‐R401 plasmid coexisted stably with the cloned incI repeats of mini‐Rtsl, suggesting that mini‐R401 RepA protein binds to incI repeats of mini‐Rtsl less efficiently than does mini‐Rtsl RepA protein.