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Production of Monoclonal Antibodies against Legionella pneumophila Serogroup 1
Author(s) -
Miyazaki Takashige,
Koga Hironobu,
Nakashima Manabu,
Tomonaga Akimitsu,
Kohno Shigeru,
Hirota Masaki,
Saito Atsushi,
Hara Kohei,
Watanabe Takeshi
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00826.x
Subject(s) - monoclonal antibody , legionella pneumophila , legionella , microbiology and biotechnology , virology , biology , antibody , monoclonal , bacteria , immunology , genetics
Four monoclonal antibodies to Legionella pneumophila Philadelphia 1 were produced by the fusion of immunized BALB/c lymphocytes to a murine myeloma cell line. Two (Lpl‐1 and Lpl‐3) of the four monoclonal antibodies reacted with 14 L. pneumophila serogroup 1 strains, and the other (Lpl‐2 and Lpl‐4) reacted with only three out of 20 strains tested. These four monoclonal antibodies did not bind to any strains of L. pneumophila serogroup 2–7 and other Legionella species. In addition, it has been shown that these monoclonal antibodies may be useful not only for subserotyping of L. pneumophila but also for retrospective diagnosis using immunopathological methods.