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Passive Immunization of Mice with Monoclonal Antibodies to Glycoprotein gB of Herpes Simplex Virus
Author(s) -
Kino Yoichiro,
Eto Tatsuo,
Ohtomo Nobuya,
Hayashi Yoshinobu,
Yamamoto Masahiro,
Mori Ryoichi
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00812.x
Subject(s) - herpes simplex virus , monoclonal antibody , virology , biology , immunization , antibody , glycoprotein , virus , herpesviridae , alphaherpesvirinae , immunology , viral disease , microbiology and biotechnology
To investigate the protective ability of monoclonal antibodies (MCAs) to viral glycoprotein in herpes simplex virus (HSV) infection, athymic nude mice were inoculated intracutaneously with HSV type‐1 (HSV‐1) in the midflank. Three hours after inoculation, one group of mice was passively immunized with one of a series of MCAs to glycoprotein gB of HSV‐1, and a control group of mice was given phosphate buffered saline alone. The control mice died within 16 days after infection, whereas the mice passively immunized with any of the MCA showed suppressed development of skin lesions. Three of six mice given MCA failed to develop any visible lesions and no HSV could be isolated from the lumbar dorsal root ganglia of these mice 60 days after the challenge. BALB/c mice were also protected from infection with HSV type 2 by passive immunization with MCA to HSV‐1 gB.