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Germinability and Heat Resistance of Spores of Clostridium difficile Strains
Author(s) -
Nakamura Shinichi,
Yamakawa Kiyotaka,
Izumi Junko,
Nakashio Satoshi,
Nishida Shoki
Publication year - 1985
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1985.tb00809.x
Subject(s) - spore , germination , lysozyme , strain (injury) , microbiology and biotechnology , biology , clostridium difficile , inoculation , clostridiaceae , brain heart infusion , clostridium , heat resistance , spore germination , bacteria , botany , horticulture , toxin , biochemistry , agar , antibiotics , genetics , materials science , composite material , anatomy
Out of 111 Clostridium difficile strains, 108 produced spores in numbers of more than 10 5 /ml and the remaining three did not produce any spores in brain heart infusion medium. The germination frequency in the medium without lysozyme varied widely from strain to strain, ranging from less than 10 −8 to 10 0 , and in 77 of the 108 strains the germination frequency was 10 −5 or less. The spores, when treated with sodium thioglycollate and then inoculated into the medium containing lysozyme, germinated in all of the 108 strains at a frequency of 10 −0.5 or more. The spores of two strains germinated at a frequency of more than 10 −0.5 in all methods. Spores of C. difficile strains were fairly highly heat‐resistant; D 100C values ranged from 2.5 to 33.5 min.