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Effect of E‐64, Thiol Protease Inhibitor, on the Secondary Anti‐SRBC Response In Vitro
Author(s) -
Amamoto Toshiro,
Okazaki Tadayasu,
Komurasaki Toshi,
Hanada Kazunori,
Omura Sadafumi
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb02949.x
Subject(s) - splenocyte , concanavalin a , biology , protease , immune system , proteases , thiol , microbiology and biotechnology , in vitro , immunology , biochemistry , enzyme
E‐64, L‐ trans ‐epoxysuccinyl‐leucylamido (4‐guanidino) butane, a specific inhibitor of thiol proteases originally isolated from the culture of a fungus, was examined in connection with the immune responses to the splenocytes of mice. In cultures of C3H/He mouse splenocytes, E‐64 and its analogues showed mitogenic activity, and some of them enhanced the lymphocyte blast transformation induced by a suboptimal concentration of concanavalin A. E‐64 caused a significant suppressive effect on the secondary anti‐SRBC responses when 7‐ or 14‐day‐primed BDF 1 mouse splenocytes were cultured with SRBC, while it induced no effect on cultured splenocytes either from mice treated with cyclophosphamide, from mice sensitized with dinitrophenyl‐Ficoll. The results with E‐64 and its close analogues revealed that their effects on the immune response roughly correlated with their inhibitory activity against thiol protease. These results suggest that a thiol protease might be involved in the process of secondary immune response in mouse splenocytes.