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Serological Cross‐Reactivities of Murine and Human Class II Antigen Determined by Murine Xeno Anti‐Human Class II Antibody
Author(s) -
Aosai Fumie,
Yui Katsuyuki,
Shigematsu Hidekazu,
Yano Akihiko
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00780.x
Subject(s) - antiserum , antibody , antigen , biology , microbiology and biotechnology , serology , molecular mass , immunology , biochemistry , enzyme
Murine anti‐human class II antibodies were shown to cross‐react with polymorphic determinants of murine class II antigens. The cross‐reacting antibodies were raised in B10.S(9R) mice by immunizing with human nylon wool adherent cells (Ad cells) from peripheral blood leukocytes. The B10.S(9R) anti‐human Ad cell antiserum bound to the molecules consisting of two chains with molecular weights of 35K and 28K dimers which were purified with a lentil‐lectin column. The B10.S (9R) anti‐human class II antiserum was also revealed to contain two distinct cross‐reacting antibodies with polymorphic determinants of murine class II antigens coded for by the I‐A subregion of the H‐2. One is specific for a determinant of class II molecules coded for by I‐A b,d,q , and the other seems to be specific for class II molecules coded for by I‐A a,k,r .