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Effect of Glucose on the Interaction of Hydrophobic Compounds with the Alanine Receptor Field of Bacillus subtilis Spores during Initiation of Germination
Author(s) -
Yasuda Yoko,
Tochikubo Kunio
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00778.x
Subject(s) - alanine , bacillus subtilis , biochemistry , hydrophobic effect , biology , binding site , spore , stereochemistry , chemistry , amino acid , microbiology and biotechnology , bacteria , genetics
Glucose interfered with the inhibitory action of hydrophobic compounds, such as n ‐octanol, diphenylamine and 2‐ tert ‐butylphenol, during L‐alanine‐initiated germination of Bacillus subtilis spores. The action of glucose on the action of the hydrophobic compounds was not competitive, and the binding affinity of glucose was not essentially affected by the hydrophobic compounds, indicating the presence of separate binding sites for glucose and the hydrophobic compounds. The binding affinity of D‐alanine, a competitive inhibitor of L‐alanine, was not affected by the hydrophobic compounds, indicating separate binding sites for D‐alanine and the hydrophobic compounds. A possible arrangement of the binding sites for glucose and for the hydrophobic compounds in relation to those for L‐ and D‐alanine on the spores is discussed.