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Composition of O‐Antigenic Lipopolysaccharides from Enterobacter cloacae
Author(s) -
White Pippa J.,
Gaston Michael A.,
Wilkinson Stephen G.
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00775.x
Subject(s) - monosaccharide , enterobacter cloacae , rhamnose , mannose , lipid a , galactose , biology , oligosaccharide , galactosamine , sugar acids , biochemistry , glucosamine , serotype , enterobacteriaceae , microbiology and biotechnology , bacteria , sugar , escherichia coli , gene , genetics
Analyses have been carried out on lipopolysaccharides (LPS) from 14 strains of Enterobacter cloacae representing different O serotypes. All of the products appeared to have a composition and architecture typical of enterobacterial LPS, but points of interest include the absence of phosphate residues from the core oligosaccharide, the presence of both L‐glycero‐D‐mannoheptose and D‐glycero‐D‐mannoheptose (ratio usually about 4: 1), and the presence in lipid A of small amounts of fatty acids with odd numbers of carbon atoms (mainly C 13 ) in addition to tetradecanoic acid and 3‐hydroxytetradecanoic acid. Monosaccharides identified as components of polymeric fractions from the LPS were glucose, galactose, mannose, rhamnose, glucosamine, galactosamine, fucosamine, and galacturonic acid. Most polymeric fractions also probably contained an O ‐acetyl substituent. Closely similar chemotypes found for the polymeric fractions from the LPS of cross‐reacting serotypes support the view that these fractions contain the O‐antigenic determinants and represent the side chains of the LPS.