Premium
Isotype Specificity and Heterogeneity of Fcγ‐Receptors on Guinea Pig Splenic B and T Lymphocytes
Author(s) -
Yamashita Toshiyuki,
Koyama Jiro
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00771.x
Subject(s) - biology , isotype , antibody , microbiology and biotechnology , rosette (schizont appearance) , receptor , guinea pig , ovalbumin , b cell , t cell , fc receptor , immunology , antigen , immune system , biochemistry , monoclonal antibody , endocrinology
The isotype specificity of Fc‐receptors for IgG (Fcγ‐Rs) on normal guinea pig splenic B and T cells was determined by a rosette assay using sheep erythrocytes sensitized with either homologous IgG1 or IgG2 anti‐sheep erythrocyte antibody [EA(IgG1) or EA(IgG2)]. Approximately 70% of the lymphocytes in the highly purified B‐cell fraction could form rosettes with EA(IgG2), and 55% of the cells with EA(IgG1). Inhibition experiments with soluble complexes of IgG1 or IgG2 antibody with ovalbumin demonstrated that approximately 20% of the EA(IgG2) rosette‐forming B cells bore the Fcγ‐R monospecific for IgG2, whereas 80% of the cells had two distinct Fcγ‐Rs simultaneously; one monospecific for IgG2 and the other bispecific for IgG1 and IgG2. The existence of a B cell bearing the Fcγ‐R monospecific for IgG1 was not definitively demonstrated in the B‐cell fraction. In the T cell‐enriched fraction, approximately 40% of the cells could form rosettes with EA(IgG2). The EA(IgG1)rosette‐forming cells, however, comprised only 6% of the total cells, indicating that most of the EA(IgG2) rosette‐forming T cells bear essentially the Fcγ‐R monspecific for IgG2 alone. The results obtained revealed that guinea pig splenic lymphocytes bear two distinct Fcγ‐Rs, which are not equally distributed on the B‐ and T‐cell populations and also on their respective subsets.