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Disappearance of Terminal Deoxynucleotidyltransferase in Human Malignant T‐Lymphoblasts Treated with a Human Alpha Interferon Preparation
Author(s) -
Takano Saeko,
Nakamura Hiromu,
Mita Haruhisa,
Nagano Yasuiti
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00768.x
Subject(s) - lymphoblast , biology , cell culture , microbiology and biotechnology , alpha interferon , interferon , cell growth , terminal deoxynucleotidyl transferase , apoptosis , immunology , virology , biochemistry , tunel assay , genetics
Human T‐lymphoblastoid cell lines RPMI 8402, MOLT‐3, and CCRF‐CEM were treated with interferon (IFN) to determine if the treatment would result in the disappearance of cellular terminaldeoxynucleotidyltransferase (TdT), a possible differentiation marker for T‐lymphocytes. Incubation of RPMI 8402 cells in the presence of IFN preparation caused a decrease in the number of TdT‐positive cells and in TdT activity of the cell extract. The inhibition of cell multiplication was dose dependent. The anticellular effect of IFN preparation was cytostatic, not cytocidal. The IFN preparation modified neither the TdT content nor proliferation of MOLT‐3 and CCRF‐CEM cell lines. The effects of IFN preparation thus varied with the cell line.