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Characterization of a Monoclonal Antibody Specific for Lipoteichoic Acid from Various Gram‐Positive Bacteria
Author(s) -
Hamada Shigeyuki,
Furuta Takushi,
Okahashi Nobuo,
Nisizawa Tosiki,
Yamamoto Toshiko,
Chiba Joe
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00757.x
Subject(s) - teichoic acid , microbiology and biotechnology , lipoteichoic acid , biology , monoclonal antibody , hemagglutination , bacteria , antibody , staphylococcus epidermidis , streptococcus pyogenes , immunodiffusion , staphylococcus aureus , immunology , genetics
A hybrid cell line, 3G6, producing monoclonal antibody (mAb) against the polyglycerophosphate (PGP) backbone of lipoteichoic acids has been derived by the polyethylene glycol‐induced fusion of mouse myeloma cells and spleen cells from mice immunized with partially purified glucosyltransferase from culture supernatant of Streptococcus mutans strain 6715. Immunodiffusion tests and ELISA revealed that the antibody reacted with purified PGP from group A Streptococcus pyogenes strain Sv as well as crude phenol‐water and saline extracts of various gram‐positive bacteria except for a few species such as biotype B S. sanguis, Micrococcus sp., and Actinomyces viscosus . Whole cells of serotype b S. mutans and Staphylococcus epidermidis were agglutinated upon addition of 3G6 mAb, while those of most other species were not significantly affected by this procedure. A hapten inhibition study showed that glycerophosphate was only a potent inhibitor of passive hemagglutination reactions between LTA coated sheep erythrocytes and 3G6 mAb.