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Isolation of Rickettsia tsutsugamushi Antigenically Different from Kato, Karp, and Gilliam Strains from Patients
Author(s) -
Tamura Akira,
Takahashi Kumiko,
Tsuruhara Takashi,
Urakami Hiroshi,
Miyamura Sadao,
Sekikawa Hiroho,
Kenmotsu Minora,
Shibata Mitsuo,
Abe Shoya,
Nezu Hiroko
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00743.x
Subject(s) - biology , virulence , rickettsiaceae , rickettsia , scrub typhus , microbiology and biotechnology , virology , orientia tsutsugamushi , rickettsiales , rickettsiosis , complement fixation test , isolation (microbiology) , strain (injury) , bacteria , antibody , virus , serology , immunology , gene , genetics , anatomy
Rickettsia tsutsugamushi strains from three recent patients of Tsutsugamushi disease in Niigata Prefecture were isolated primarily in mice and then in L cell cultures. By this procedure, low virulent strains to mice, as well as high virulent ones, could be isolated and cultivated serially in L cell cultures, suggesting the usefulness of L cells for isolation of this species of rickettsia. Each newly isolated strain was identified as a member of R. tsutsugamushi from the results of cross immunological tests and morphological observation. On the other hand, it was recognized that one of these rickettsiae showed immunological properties distinguishable from the prototype strains of Kato, Karp, and Gilliam by the cross complement fixation test, and also had low virulence in mice.