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Polysaccharides with Sulfate Groups Are Human T Cell Mitogens and Murine Polyclonal B Cell Activators (PBAs)
Author(s) -
Sugawara Isamu,
Ishizaka Shigeaki
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00738.x
Subject(s) - fucoidan , chondroitin sulfate , sulfation , sulfate , biochemistry , microbiology and biotechnology , chondroitin sulfate proteoglycan , polysaccharide , biology , chemistry , glycosaminoglycan , organic chemistry
In our previous paper, we reported that various types of carrageenan, dextran sulfate and fucoidan, which are sulfated homopolysaccharides with high molecular weights, were human T cell mitogens and murine polyclonal B cell activators (PBAs) and that heparin, a sulfated heteropolysaccharide, was a very weak human mitogen and mouse PBA. Here we used cellulose sulfate (Mr 7–9 × 10 3 ), dextran sulfate with two different low molecular weights (Mr 5 × 10 3 and 8 × 10 3 ), two different condroitin sulfates (Mr 3.5 × 10 4 ), polyvinyl sulfate and polygalacturonic acid to investigate mitogenic activities of polysaccharides in detail. The following results were obtained. 1. Low‐molecular‐weight sulfated homopolysaccharides, dextran sulfate and cellulose sulfate, were very weak or not human T cell mitogens. However, they were better murine PBAs. 2. Sulfated hetero‐polysaccharides, chondroitin 4‐sulfate and chondroitin 6‐sulfate, hardly induced mitogenic changes in human T cells and mouse B cells, even though the molecular weight of these substances was more than 1 × 10 4 . 3. There were no other polymers examined so far which activated both human T cells and murine B cells. The relationship among molecular size, sulfate groups and lymphocyte activation is discussed in detail.