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Development of a New Cell System for the Infectivity Assay of Dengue Viruses: Plaque Formation and Virus Growth of Prototype and Wild‐Type Dengue Virus Strains in a Newly Established Cell Line, GK
Author(s) -
Nawa Masaru
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00732.x
Subject(s) - dengue virus , virology , biology , infectivity , virus , dengue fever , strain (injury) , wild type , cell culture , antibody dependent enhancement , plaque forming unit , virus quantification , microbiology and biotechnology , mutant , genetics , gene , anatomy
A newly established cell line, GK, derived from the kidney tissue of Mongolian gerbils, produced plaques by infection of prototype and wild‐type dengue virus strains. Both prototype and wild strains of type 2 virus grew in GK cells and formed plaques at 35.5 C and at 31 C, while types 1, 3, and 4 wild strains grew and formed plaques only at 31 C. In GK cells, plaque formation and the growth of dengue viruses depended on the high (35.5 C) and low (31 C) incubation temperatures. Virus yields in GK cells of all the 14 dengue virus strains tested, including four prototype and ten wild‐type viruses, were 5 to 1,000‐times lower than those in C6/36 cells. After five serial passages in GK cells, types 2, 3, and 4 prototype viruses and type 2 wild strain increased virus yields, and one strain of prototype virus and one strain of wild‐type virus decreased mouse neurovirulence.