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Calcium Behavior in Endotoxin‐Poisoned Mice: Especially Calcium Accumulation in Mitochondria
Author(s) -
Sakaguchi Shuhei,
Abe Hiroharu,
Sakaguchi Osamu
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00704.x
Subject(s) - endocrinology , calcium , medicine , trifluoperazine , biology , verapamil , calmodulin , antagonist , antiglucocorticoid , glucocorticoid , receptor , glucocorticoid receptor
A possible role of intracellular Ca 2+ and participation of calmodulin in cellular metabolism in endotoxin‐poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18–48 hr after endotoxin injection than in the control mice. On the other hand, the levels of serum calcium in the poisoned mice were about 20% lower at 18 hr than in the controls. The serum calcium levels in mice injected with 50 and 100 μg of endotoxin showed no dose‐response effect, but a dose‐response effect was observed at a dose of 200–400 μg. The serum Ca 2+ levels in endotoxin‐tolerant mice were similar to those in the control mice. The levels in mice injected with glucocorticoid‐antagonizing factor mice were about 14% lower at 3 hr than in the controls. The mice fed a vitamin D3‐ and calcium‐free diet showed a higher mortality rate in the early stage (12–18 hr) of endotoxication than that of the mice fed a normal diet. The lipid peroxide levels and Ca 2+ ‐ATPase activity in the liver mitochondria fraction in endotoxin‐poisoned mice showed a higher level than those of the control mice. There was little or no difference in the levels of serum glucose between the mice injected with calmodulin antagonist (trifluoperazine, TFP) plus endotoxin and those given endotoxin alone. However, the liver glycogen levels in TFP plus endotoxin‐treated mice were markedly higher than that in mice given endotoxin alone. Furthermore, calcium antagonist (verapamil) plus endotoxin‐treated mice had about a 40% higher survival rate after 72 hr than those given endotoxin alone. The findings suggest that there is a possibility of participation of the Ca 2+ ‐calmodulin system in carbohydrate metabolic disorders during endotoxemia and that the changes in intracellular Ca 2+ may result in various metabolic disorders.