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Immunoglobulin Classes of Anti‐Sendai Virus Antibody Detected by ELISA in Infected Nude Mouse Serum
Author(s) -
Iwai Hiroshi,
Yamaguchi Ryoji,
Otsuka Yuko,
Ueda Katsumoto,
Saito Muneo
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00699.x
Subject(s) - sendai virus , antiserum , antibody , biology , virology , microbiology and biotechnology , sephadex , virus , antigen , immunoglobulin g , immunology , enzyme , biochemistry
Sendai virus‐infected nude mouse sera obtained on the seventh day after infection or later, in which anti‐Sendai virus antibodies were undetectable by hemagglutination‐inhibition and neutralization tests, were found to be reactive with the virus antigen by ELISA using horseradish peroxidase‐conjugated anti‐mouse IgG rabbit IgG. The reactivity was blocked by rabbit anti‐Sendai virus antiserum and was not observed against influenza virus which served as a control antigen. Anti‐Sendai virus antibody activity of fractions from Sephadex G‐200 gel filtration was detected in the IgM fraction when anti‐mouse μ chain‐specific antiserum was used and in both IgG and IgM fractions when heavy and light chain‐specific anti‐mouse IgG serum was employed in ELISA. ELISA of the fractions from protein A‐Sepharose affinity chromatography of Sendai virus‐infected nude mouse sera showed that the eluates at pH 6.0 and pH 3.5 contained IgG1 and IgG2b anti‐Sendai virus antibodies, respectively, and that the eluate at pH 4.5 contained both IgG2a and IgG3 antibodies.