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4‐Hydroxy‐3‐Nitrophenyl (NP) Acetyl‐Hapten Specific Lymphocyte Proliferation
Author(s) -
Okuda Kenji,
Saito Harukazu,
Aihara Yukoh,
Minami Mutsuhiko,
Tadokoro Ichiro
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00698.x
Subject(s) - hapten , biology , immunology , lymphocyte subsets , lymphocyte , t lymphocyte , t cell , immune system , antigen
Hapten specific T cell proliferation was induced in several strains of mice. When lymph node T cells from 4‐hydroxy‐3‐nitrophenyl acetyl‐keyhole lympet hemocyanin (NP‐KLH)‐primed mice were stimulated in vitro with NP‐polymer glutamic acid‐lysine‐phenyl alanine (NP‐GL φ ) or NP‐ovalbumin (NP‐OVA), they displayed a good level of proliferative responses. It was observed that NP‐GL φ could induce NP‐hapten specific proliferation even with NP‐KLH lymphocytes from GL φ non‐responder strains. NP‐KLH primed lymphocytes from C57BL/6 (H‐2 b , Igh‐1 b ), CKB (H‐2 k , Igh‐1 b ), CWB (H‐2 b , Igh‐1 b ), and B10.BR (H‐2 k , Igh‐1 b ) mice showed good proliferative responses to both 4‐hydroxy‐5‐iodo‐3‐nitrophenyl (NIP) acetyl‐GL φ and NIP‐OVA antigens. However, NP‐KLH primed lymphocytes from C3H/He (H‐2 k , Igh‐1 j ) and C3H. SW (H‐2 b , Igh‐1 j ) mice displayed poor proliferative responses to NIP‐GL φ and NIP‐OVA antigen. These results suggested that the gene coding for the NIP‐cross‐reaction might be mapped in the Ig heavy‐chain linked locus.