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Characterization and Pathogenicity of Hemolysis Mutants of Mycoplasma pneumoniae
Author(s) -
Yayoshi Masumi,
Araake Minako,
Hayatsu Eizo,
Kawakubo Yasuaki,
Yoshioka Morimasa
Publication year - 1984
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1984.tb00682.x
Subject(s) - hemolysis , mycoplasma pneumoniae , hamster , biology , microbiology and biotechnology , mycoplasma , mutant , strain (injury) , pathogenesis , lung , mesocricetus , immunology , genetics , gene , medicine , pneumonia , anatomy
Abstract Hemolysis mutants were produced by treating Mycoplasma pneumoniae FH‐P24 strain with N ‐methyl‐ N ‐nitro‐nitrosoguanidine and were classified into three different groups. The first group of mutants, strains P24‐L1, L2, and L11, showed wide and clear hemolytic zones. Their attachment ability to erythrocytes of various animals and to hamster lung cells were the same as those of the parent strain. The second group, strain P24‐S1, showed non‐hemolysis and non‐hemadsorption, but retained the attachment ability to lung cells, although not to erythrocytes. The third group, strain P24‐S11, was non‐hemolytic, had completely lost the attaching ability, and did not proliferate in vivo . Strains in the first group produced significant microscopic pneumonic lesions in hamsters while strain P24‐S1 produced milder lung lesions. Strain P24‐S11 did not cause any lung lesions, and organisms were not recovered from the lungs of hamsters. The attachment of M. pneumoniae to respiratory epithelium as a cause of infection and the existence of a relationship between the hemolytic abilities of the organisms and histopathogenicity in the hamster lung tissue were further supported by the present data. It was also shown that the use of hemolysis mutants is useful for the elucidation of pathogenesis in mycoplasmal infections.