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Stimulation of Locomotion of Peripheral Blood Monocytes by Human Plasma Fibronectin
Author(s) -
Yonemasu Kunio,
Nakanishi Akira,
Sasaki Takako,
Kashiba Syuzo
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb03590.x
Subject(s) - fibronectin , chemotaxis , motility , monocyte , stimulation , biology , in vitro , colchicine , in vivo , microbiology and biotechnology , immunology , endocrinology , biochemistry , extracellular matrix , receptor , genetics
The motility of human peripheral blood granulocytes and monocytes in response to human plasma fibronectin was quantified by an in vitro assay using blind‐well chemotaxis chambers. Purified fibronectin under nondenaturing conditions produced increased migration of granulocytes only at concentrations higher than 100 nm, and induced increased chemotactic and random locomotion of monocytes at concentrations higher than 0.1 nm. The monocyte migration‐inducing activity of fibronectin was concentration dependent, and was strongly inhibited by low concentrations of colchicine (100 nm–100 μm). These findings suggest the possibility that plasma fibronectin serves as a chemotactic stimulus for monocytes in vivo and attracts these cells to sites of microscopic tissue injury where plasma fibronectin is deposited.