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Biological Activities of Guinea Pig Mitogenic Factor from Immune Lymphocytes Stimulated with Antigen
Author(s) -
Iribe Hideaki,
Koga Toshitaka
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb03568.x
Subject(s) - biology , lymphokine , antigen , guinea pig , lymphoblast , monokine , growth factor , immune system , microbiology and biotechnology , t lymphocyte , immunology , endocrinology , medicine , cell culture , cytokine , interleukin , biochemistry , receptor , genetics
Mitogenic factor was produced by sensitized guinea pig lymph node cells stimulated with a specific antigen. Both T lymphocytes and macrophages were required for the production of this factor. The culture supernatant of lymphocytes containing the mitogenic factor exhibited a strong helping effect on the proliferative response of T lymphocytes to phytohemagglutinin (PHA). Mitogenic factor and the factor with the helping activity coeluted in the molecular weight range of 25,000‐35,000 daltons in gel filtration. Furthermore the fraction containing mitogenic factor was found to support the proliferation of lymphoblasts induced by PHA or antigen, suggesting that the mitogenic factor may be the guinea pig equivalent of T cell growth factor (TCGF) reported in the mouse, rat, and human. On the other hand, the T cell‐activating monokine of the guinea pig, possessing the helping activity for the proliferative response of T lymphocytes to PHA, never exhibited TCGF‐like activity.