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Incompatibility Group K Plasmids in Bacteria Isolated from a Urinary Tract Infection
Author(s) -
Araki Yasuhisa,
Inoue Matsuhisa,
Mitsuhashi Susumu
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb03560.x
Subject(s) - plasmid , kanamycin , microbiology and biotechnology , biology , ampicillin , gentamicin , citrobacter freundii , klebsiella pneumoniae , streptomycin , aminoglycoside , bacteria , antibiotics , escherichia coli , genetics , gene
Citrobacter freundii and Klebsiella pneumoniae were concurrently isolated from a patient with a urinary tract infection. Transferable drug resistant plasmids were isolated from both strains, pMS434 and pMS435. These plasmids belonged to incompatibility group K and both carried genes governing resistance to various aminoglycoside antibiotics, i.e ., kanamycin, gentamicin C complex, streptomycin, and 3′, 4′‐dideoxykanamycin B, in addition to those governing resistance to sulfanilamide and ampicillin. They inactivated kanamycin, gentamicin C complex and 3′,4′‐dideoxykanamycin by adenylylation and kanamycin by phosphorylation. Electron microscopic observations disclosed that the molecular weights of the plasmids were about 67.8 megadaltons. These results indicated the similarity in genetic constitution of the two plasmids. This was the second isolation of incompatibility group K plasmids, following that reported by Hedges and Datta.