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Sporulation Promoting Factor in Vegetative Cells of Bacillus subtilis
Author(s) -
Hachisuka Yoetsu,
Imura Hiromi,
Nakata Akemi,
Hattori Emiko
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb02935.x
Subject(s) - bacillus subtilis , spore , biology , size exclusion chromatography , nutrient agar , agar , fractionation , bacillales , microbiology and biotechnology , chromatography , biochemistry , bacteria , chemistry , enzyme , genetics
Abstract A simple experimental system for detection of sporulation promoting factors was presented. This system showed that there was a sporulation promoting factor in the vegetative cells of Bacillus subtilis cultivated on nutrient agar for 9 hr (at stage T 0 ). The factor was partially purified from the sonicate of vegetative cells by ethanol fractionation, gel filtration, chromatography and preparative gel electrophoresis, and it was identified as manganese‐containing protein.