Premium
T Cell‐Activating Monokines in Guinea Pigs: Comparison of High and Low Molecular Weight Factors
Author(s) -
Sakamoto Shigeru,
Onoue Kaoru,
Ohishi Masamichi
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb02932.x
Subject(s) - monokine , biology , guinea pig , microbiology and biotechnology , endocrinology , immunology , medicine , interleukin , cytokine
Guinea pig monokines produced by lipopolysaccharide‐stimulated peritoneal macrophages were found in high (50,000–80,000) and low (10,000–30,000) molecular weight (m.w.) fractions by gel filtration. Both showed enhancing activity on the proliferative response of guinea pig and mouse thymocytes to PHA, but the high m.w. (65K) monokine was much more efficient than the low m.w. (15K) monokine in enhancing the response of lymph node T cells to PHA, suggesting its importance in the activation of peripheral T cells. The 65K monokine was coeluted with BSA present in the culture medium by DEAE‐cellulose chromatography, but was clearly separated from it by hydroxylapatite chromatography. The immunoadsorption experiment with anti‐BSA‐coupled gel also indicated that 65K monokine is not a complex of low m.w. monokine with BSA. Our series of studies showed that most monokine activities were always found in the 65K fraction in guinea pigs. Thus, in guinea pigs, the 65K component appears to constitute a major class of T cell‐activating moonkines.