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Mouse Alloantibodies Capable of Blocking Cytotoxic T Cell Function
Author(s) -
Kojima Masaharu,
Taniguchi Masaru,
Shinohara Nobukata
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb02930.x
Subject(s) - cytotoxic t cell , antibody , biology , antiserum , monoclonal antibody , antigen , major histocompatibility complex , immunology , blocking antibody , epitope , microbiology and biotechnology , in vitro , biochemistry
Blocking of cell‐mediated lympholysis (CML) by anti‐Lyt‐2 antibodies was compared with that by anti‐H‐2 antibodies which most likely inhibit CML by blocking antigen recognition by cytotoxic T lymphocytes (CTLs). Both antibodies were shown to inhibit the early Mg 2+ ‐dependent process of killing. Moreover, the anti‐H‐2‐sensitive event was found to be reversible by the antibody as was the case with the anti‐Lyt‐2‐sensitive event, suggesting that the two antibodies block the same event taking place during the Mg 2+ ‐dependent stage. Both types of antibody were also shown to be capable of inhibiting the phytohemagglutinin (PHA)‐mediated nonspecific killing activity of CTLs. However, in the case of anti‐Lyt‐2 antibodies, available monoclonal antibodies failed to inhibit PHA‐mediated killing whereas conventional antisera did. The results thus suggest multiplicity and heterogeneity of Lyt‐2 determinants or the existence of multiple products of Lyt‐2 ‐linked genes. In addition, an anti‐H‐2 antiserum also exerted a specific inhibitory effect on PHA‐mediated killing. Thus there appears to be a general requirement for involvement of the Lyt‐2 molecules on CTLs and major histocompatibility complex (MHC) products on the target cells. The implications of these observations are discussed.