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Preparation of Membrane Fraction from Herpes Simplex Virus‐Infected Cells which Induce Cytotoxic T Lymphocytes
Author(s) -
Hitsumoto Yasuo,
Sonoda Shunro,
Okuyama Masaaki,
Miki Yoshiharu,
Utsumi Sayaka
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb00641.x
Subject(s) - ctl* , cytotoxic t cell , biology , herpes simplex virus , immunogen , virus , virology , membrane , microbiology and biotechnology , immunology , in vitro , biochemistry , antibody , monoclonal antibody
The immunogenic capacity of herpes simplex virs (HSV)‐infected cells and their subcellular membrane fractions was investigated by assessing the anti‐HSV cytotoxic T lymphocyte (CTL) response in cultures of spleen lymphocytes from HSV‐primed BALB/c mice. Methylchloranthrane‐induced fibrosarcoma (Meth A) cells infected with HSV (HSV‐Meth A) were fixed either with glutaraldehyde or by heating at 56 C to preserve their immunogenic competence and then used as a stimulator. Microsomes and plasma membranes were prepared from HSV‐Meth A and their immunogenic activities were determined. Though the recovery of stimulatory activity in the plasma membrane fraction was half of that in the microsome fraction, the activity in the former was much more stable than in the latter and the plasma membrane fraction proved to be well qualified as an immunogen for anti‐HSV CTL induction. Upon purification, the specific activity of the membrane fraction, on the basis of protein concentration, increased 43‐fold.