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Effect of High Salt Treatment on Influenza B Viral Protein Synthesis in MDCK Cells
Author(s) -
Tsurumi Tatsuya,
Aoki Hiizu,
Nishiyama Yukihiro,
Shibata Motohiro,
Maeno Koichiro,
Seo Hisao
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb00613.x
Subject(s) - biology , translation (biology) , vesicular stomatitis virus , messenger rna , protein biosynthesis , virus , viral protein , virology , orthomyxoviridae , in vitro , influenza a virus , microbiology and biotechnology , biochemistry , gene
Abstract Based on the information that high salt inhibits the initiation of cellular mRNA translation which depends on the function of the 5′‐terminal structure of mRNA, we compared the effect of high salt on translation of host cellular mRNAs and influenza viral mRNAs, both of which are of 5′‐terminal structure. Brief exposure of influenza B virus‐infected MDCK cells to high salt medium resulted in a dose‐dependent inhibition of viral polypeptide synthesis as well as of cellular polypeptide synthesis, but it had less effect on synthesis of viral polypeptides, particularly nonstructural protein (NS). Under these conditions the Na + content of the infected cells was significantly increased. A similar salt effect on in vitro translation of viral and cellular mRNAs extracted from infected cells was also observed. There was no significant difference in sensitivity to hypertonic block of in vivo translation of influenza viral mRNAs and vesicular stomatitis virus mRNAs, the latter of which possess a virus‐directed structure at the 5′‐terminus.