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Cytotoxin Production by Clostridium sordellii Strains
Author(s) -
Nakamura Shinichi,
Tanabe Naomi,
Yamakawa Kiyotaka,
Nishida Shoki
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb00611.x
Subject(s) - antitoxin , biology , toxin , clostridium difficile , microbiology and biotechnology , baby hamster kidney cell , clostridium difficile toxin a , incubation , clostridium , clostridiaceae , clostridium difficile toxin b , hamster , virology , bacteria , virus , antibiotics , biochemistry , genetics
A total of 55 strains of Clostridium sordellii , 21 lethal toxin‐positive and 34 lethal toxin‐negative, were tested for cytotoxin production in brain heart infusion medium supplemented with 0.2% Na 2 HPO 4 (m‐BHI) and cooked‐meat‐glucose (CMG) medium using baby hamster kidney (BHK‐21/WI‐2) cells as indicator cells. The m‐BHI medium was preferred to CMG medium and 24 hr of incubation was sufficient for cytotoxin production. Nineteen of the 21 toxigenic strains were also cytotoxigenic, and the strength of the cytotoxigenicity was approximately parallel with that of the lethal toxigenicity. Clostridium difficile antitoxin neutralized C. sordellii cytotoxin and also C. sordellii antitoxin neutralized C. difficile cytotoxin.