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Plaque Formation by Tyzzer's Organism in Primary Monolayer Culture of Mouse Hepatocytes
Author(s) -
Kawamura Seiji,
Taguchi Fumihiro,
Fujiwara Kosaku
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb00600.x
Subject(s) - biology , microbiology and biotechnology , antiserum , organism , inoculation , virulence , host (biology) , virus quantification , cell culture , antibody , immunology , biochemistry , genetics , gene , paleontology , ecology
Tyzzer's disease organism propagated on primary monolayer cultures of mouse hepatocytes and produced definite plaques. In phase contrast microscopy, the organisms were motile in the plaques. Plaque formation was inhibited by antiserum. After serial plaque cloning the organisms still had virulence in mice. To establish a standard plaquing procedure, factors affecting plaque formation were studied. The critical factors in plaque formation were the culture period of host cells before inoculation, medium for suspending the organisms, and temperature and time of infection. A 24 to 36 hr‐preculture of host cells and trypticase soy broth (BBL) as the suspending medium gave the best results. The optimal conditions for infection were 37 C for 90 min. The plaquing efficiency was higher when a larger volume of inoculum was applied to host cell monolayers, suggesting that the organism played an active role in the initial stage of infection.