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Inhibition of Macrophage DNA Synthesis by Immunomodulators
Author(s) -
Nagao Shigeki,
Tanaka Atsushi
Publication year - 1983
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1983.tb00596.x
Subject(s) - muramyl dipeptide , thymidine , lipopolysaccharide , lymphokine , macrophage , dna synthesis , biology , glucosamine , macrophage activating factor , exudate , microbiology and biotechnology , pharmacology , immunology , dna , biochemistry , immune system , in vitro , botany
Oil‐induced guinea pig peritoneal exudate macrophages were found to incorporate actively [ 3 H]thymidine without any tissue fluids such as conditioned medium, lymphokines or inflammatory tissue exudates. The [ 3 H]thymidine incorporation was markedly suppressed by macrophage stimulants such as muramyl dipeptide (MDP) or bacterial lipopolysaccharide (LPS), while glucosamine incorporation was simultaneously increased by these stimulants. The degree of suppression of thymidine incorporation depended on the cell density, the concentrations of the stimulants, and sera or culture media used. The exposure of macrophages to MDP for 30 min was sufficient to cause significant suppression.