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Adhesion of Haemophilus paragallinarum to Cultured Chicken Cells
Author(s) -
Ueda Susumu,
Nagasawa Yoji,
Suzuki Taeko,
Tajima Masanori
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00250.x
Subject(s) - biology , virulence , microbiology and biotechnology , adhesion , haemophilus , cytoplasm , bacteria , strain (injury) , anatomy , chemistry , genetics , gene , organic chemistry
Adhesion of Haemophilus paragallinarum to chicken embryo fibroblasts (CEF) in vitro was investigated in correlation with its virulence. Under the scanning electron microscope, the organisms were seen adhering to CEF exposed to the bacteria. By transmission electron microscopy, the organisms appeared to attach to the plasma membrane of CEF by their fuzzy material. They were enclosed in membrane‐limited vesicles and appeared morphologically intact. Specific fluorescence was seen in the cytoplasm of CEF in later stages of infection. There was a good correlation between pathogenicity of the organisms for chickens and their ability to adhere to CEF. This correlation was reinforced by the fact that the ability of a pathogenic strain to adhere to the mucosal surface of the chicken trachea was demonstrated by scanning electron microscopy.