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Delayed‐Type Hypersensitivity to Hen Egg‐White Lysozyme: I. The Surface Phenotypes of Suppressor T Cells Induced by Intravenous Injection of Lysozyme‐Modified Spleen Cells, and Their Moleculor Interactions
Author(s) -
Okuda Kenji,
Ishii Norihisa,
Ikezawa Zenroh,
Takahashi Toyozoh,
Arai Katsuji,
Tadokoro Ichiro
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00236.x
Subject(s) - lysozyme , biology , antibody , antiserum , microbiology and biotechnology , idiotype , antigen , receptor , egg white , spleen , immunology , monoclonal antibody , biochemistry
Suppressor T cells (Ts) induced by lysozyme‐modified syngeneic lymphocytes were characterized. Hen egg‐white lysozyme (HEL)‐specific delayed‐type hypersensitivity (DTH) was suppressed when HEL‐induced Ts were transferred into naive mice. These HEL‐induced Ts had surface markers of both Thy‐1 antigen, and I‐J gene products. The suppression of HEL‐specific DTH was greatly increased, when these Ts had been enriched with HEL‐coated petri dishes. Isolated anti‐HEL antibodies from BIO.BR or A/Sn mice were inoculated into rabbits to induce anti‐cross‐reactive idiotype (CRI) antibodies. The rabbit antisera were extensively absorbed with normal B10.BR or A/Sn immunoglobulins (Igs) and MOPC 104E ascites Igs to render them idiotype (Id) specific. Using these anti‐CRI antibodies, we observed that these Ts possessed Id receptors on their cell surface. Results of both fluorescence techniques and cytotoxicity tests revealed that about 10% of the enriched T cells containing these Ts were Id positive. Moreover, these enriched T cells were substantially killed by anti‐ I‐J antiserum plus complement. However, this killing was completely blocked by HEL antigen. These results suggest that both Id receptors and I‐J gene products might be forming the same molecular complexes or might coexist in the vicinity of the molecule.