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Mechanisms of Resistance in Escherichia coli to the Sideromycin Antibiotic No. 216: Isolation and Characterization of the Resistant Mutants
Author(s) -
Miyakawa Satohide,
Noto Takao,
Okazaki Hiroshi
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00235.x
Subject(s) - biology , microbiology and biotechnology , escherichia coli , isolation (microbiology) , antibiotics , mutant , antibiotic resistance , genetics , gene
Escherichia coli easily developed resistance to a new antimicrobial agent of the sideromycin group, No. 216, by spontaneous mutation. Most of the No. 216‐resistant mutants tested proved not to be cross‐resistant to E. coli phages T1, T5, and φ80. On the other hand, these phage‐resistant mutants were cross resistant to No. 216. The initial site for binding of No. 216 to the sensitive cells was located, at the ton A gene product (Ton A‐protein) of the outer membrane. However, unlike the phage‐resistant mutants, ton A protein (78K‐protein) in most No. 216‐resistant mutants was intact and these mutants were possessed a particular 87K protein in the outer membrane. It is suggested that No. 216 is taken up by ton A protein and then penetrates into the cell by way of a particular transport system and that a highly mutable portion may exist in this reaction system.