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Integration of Bovine Leukemia Virus DNA in the Genomes of Bovine Lymphosarcoma Cells
Author(s) -
Onuma Misao,
Sagata Noriyuki,
Okada Kosuke,
Ogawa Yasuki,
Ikawa Yoji,
Oshima Kanichi
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00227.x
Subject(s) - provirus , bovine leukemia virus , biology , southern blot , virology , restriction enzyme , complementary dna , genome , microbiology and biotechnology , dna , virus , restriction map , nucleic acid thermodynamics , genetics , gene , plasmid , base sequence
Abstract Integration of bovine leukemia virus (BLV) in the genomes of infected cells was investigated in cattle with enzootic bovine leukosis (EBL) and sporadic bovine leukosis (SBL). Southern blot hybridization of BLV cDNA to Eco RI and Xba I restriction fragments of EBL tumor DNAs revealed that: 1) one to four or more copies of proviral DNA were integrated per genome; 2) the restriction pattern of the integrated proviral DNA was the same in two or three different tumors from the same animals; and 3) different patterns were observed among tumors from four different animals. These findings suggest the monoclonal origin of different tumors in an individual animal and the existence of multiple chromosomal integration sites of BLV provirus. DNAs from several SBL tumors were also analyzed with the same restriction enzymes, but with both representative and cDNA 3′‐enriched's of BLV RNA. No hybridization bands reactive with representative BLV cDNA could be detected, while several bands appeared to hybridize with cDNA 3′‐enriched .