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R483 and F Plasmid Genes Promoting RNA Degradation: Comparative Restriction Mapping
Author(s) -
Akimoto Shigeru,
Ohnishi Yoshinari
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00224.x
Subject(s) - ecori , pbr322 , biology , plasmid , bamhi , gene , restriction map , microbiology and biotechnology , genetics , nucleic acid , dna , restriction fragment , rna , restriction site , restriction enzyme
The gene p romoting nucleic‐acid d egradation ( pnd ) on IncI a plasmid R483 was cloned into pBR322. It is located on a 0.85 kilobase (kb) Eco RI‐ Sal I fragment and is close to Tn 7 . The pnd gene has similar properties to the srnB gene on the F plasmid. A cleavage map of the 0.85 kb pnd fragment was constructed and compared with that of the 1.18 kb Eco RI‐ Bam HI fragment containing the srnB gene. These two regions showed marked heterogeneity as evidenced by their distinctly different restriction maps. This result suggests separate paths of evolution of the two genes for stable RNA degradation.