Premium
Effect of Interferon on the Production of HBsAg and Induction of an Antiviral State in Human Hepatoma Cell Line PLC/PRF/5
Author(s) -
Nakajima Yukio,
Kuwata Tsuguo,
Tomita Yoshimi,
Okuda Kunio
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00213.x
Subject(s) - hbsag , vesicular stomatitis virus , biology , cell culture , virology , interferon , microbiology and biotechnology , alpha interferon , virus , hepatitis b virus , genetics
The effects of human α and β interferons (IFN) on the production of HBsAg by PLC/PRF/5 cells, an HBsAg‐producing human hepatoma cell line, were studied in the exponential and stationary phases of cell growth. When exponential phase cells were treated with 100 or 1,000 U of IFN per ml for 48 hr, the amount of HBsAg in the culture medium decreased. The number of cells and the synthesis of DNA and proteins were also reduced by the IFN treatment. These results suggested that IFN did not affect the production of HBsAg specifically in exponential phase cells. When cells in the stationary phase were similarly treated with IFN, HBsAg production was not inhibited nor did the number of cells decrease. To examine the antiviral state induced by IFN in PLC/PRF/5, induction of 2′5′‐oligo(A) synthetase and susceptibility to two kinds of viruses were examined. The 2′5′‐oligo(A) synthetase activity was increased in an IFN‐dose dependent manner. Susceptibility to vesicular stomatitis virus (VSV) and encephalomyocarditis virus (EMCV) was decreased by treatment with 10 and 100 U of IFN per ml for 20 hr. It was concluded that IFN‐α and IFN‐β induce 2′5′‐oligo(A) synthetase and the antiviral state, but do not inhibit HBsAg production by PLC/PRF/5 cells.