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Appearance of Enterotoxigenic Escherichia coli in Piglets with Diarrhea in Connection with Feed Changes
Author(s) -
Shimizu Mikio,
Terashima Toyoaki
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00200.x
Subject(s) - diarrhea , microbiology and biotechnology , enterotoxigenic escherichia coli , escherichia coli , enterotoxin , biology , ampicillin , feces , streptomycin , heat stable enterotoxin , antibiotics , medicine , biochemistry , gene
Twelve weaned piglets (3‐week‐old) were divided into three groups according to the time of feed change and observed for diarrhea during the time they were 3 to 8 weeks of age. A total of 553 strains of Escherichia coli were isolated from rectal fecal samples and examined for heat‐labile (LT) and heat‐stable (ST) enterotoxins, pilus antigens (K88, K99, and 987P), hemolysin (Hly), raffinose utilization (Raf) and drug resistance. Enterotoxigenic and/or hemolytic E. coli strains appeared in the rectal feces of 5‐ to 6‐week‐old piglets with diarrhea in connection with feed change and changing temperatures. Most of the isolates showed multiple drug resistance to sulfonamides (Sa), streptomycin (Sm), chloramphenicol (Cp), tetracycline (Tc), kanamycin (Km), spectinomycin (Sp), ampicillin, and/or mercury. Enterotoxigenic E. coli isolates represented four phenotypes: K88+.LT+.Hly+.Raf+.(SaSmCpTcKmSp) (12 strains), K99+.ST+.Raf+.(TcKm) (7 strains), ST+.Raf+.(TcKm) (7 strains), and ST.+(SaSmKm) (25 strains). The drug resistance determinants were transferable concurrently and some of them mobilized the determinants for K88, LT, Hly, and Raf to an E. coli C strain.