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Immunochemical Characterization of Candida albicans Cell Wall Antigens: Specific Determinant of Candida albicans Serotype A Mannan
Author(s) -
Suzuki Motofumi,
Fukazawa Yoshimura
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00189.x
Subject(s) - mannan , candida albicans , mannose , biology , corpus albicans , biochemistry , antigen , microbiology and biotechnology , polysaccharide , genetics
We investigated the chemical structure of the specific determinant in the mannan of Candida albicans M‐1012 (serotype A) strain. Acetolysis of the mannan, obtained by alkali extraction and purified as the copper complex, gave mannose and six oligosaccharides (from di‐ to hexasaccharide) and a small amount of a heptasaccharide. We examined the inhibition by these oligosaccharides up to hexaose of the precipitin reaction between anti‐factor 6 serum specific for serotype A and homologous mannan, and found that the mannohexaose was the most effective inhibitor. These, and results obtained by proton magnetic resonance (PMR) spectroscopy, methylation analysis, and other structural studies, suggest that the main component of this hexaose consists of one terminal α(1–3) linkage in addition to four α(1–2) linkages, and that this α(1–3)‐containing mannohexaose may be responsible for the specificity of antigenic factor 6. Further results obtained by analyses of polarimetry, PMR spectroscopy, and chromium trioxide oxidation‐methylation of C. albicans M‐1012 mannan and its acid‐degraded mannans suggest that the mannan has a β‐linkage in addition to α‐linkages, and that the mode of the β‐linkage is mainly (1–6) linkage. Further evidence obtained by Smith degradation‐methylation analysis and by quantitative precipitin reactions of intact and acid‐degraded mannan suggests that the antigenic determinant of antigenic factor 6 may be bound, via the β(1–6) linkage, to C‐6 of mannose residues involved in oligosaccharide side chains of serotype A mannan.