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Purification of Rickettsia tsutsugamushi by Percoll Density Gradient Centrifugation
Author(s) -
Tamura Akira,
Urakami Hiroshi,
Tsuruhara Takashi
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00181.x
Subject(s) - percoll , biology , budding , intracellular , differential centrifugation , infectivity , rickettsia , centrifugation , cell membrane , intracellular parasite , microbiology and biotechnology , virology , membrane , biochemistry , virus
Purification of Rickettsia tsutsugamushi has been achieved by Percoll density gradient centrifugation. The microorganisms purified showed good retention of infectivity and intracellular morphology. Budding rickettsiae in the egressing stage and intracellular rickettsiae in the multiplying process were harvested separately and purified by this technique. In electron microscopic observations, the intracellular rickettsiae obtained were surrounded with double membrane‐layers of cell wall and cell membrane, and the budding rickettsiae were enveloped with an additional outermost membrane which may have originated from host cell membrane obtained in the budding process.