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Modulation of Immune Response by Bacterial Lipopolysaccharide (LPS): Roles of Macrophages and T Cells in In Vitro Adjuvant Effect of LPS on Antibody Response to T Cell‐Dependent and T Cell‐Independent Antigens
Author(s) -
Uchiyama Takehiko
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00173.x
Subject(s) - spleen , lipopolysaccharide , macrophage , biology , immune system , microbiology and biotechnology , ficoll , adjuvant , t cell , in vitro , immunology , biochemistry , peripheral blood mononuclear cell
The roles of macrophages and T cells in the adjuvant effect of lipopolysaccharide (LPS) were studied. In vitro anti‐trinitrophenyl (anti‐TNP) antibody responses to TNP‐Ficoll and TNP‐keyhole limpet hemocyanine (TNP‐KLH) in spleen cells of C57BL/6 mice showed the most enhancement, when LPS was added to cultures at 1 μ g/ml 48 hr after culture was started. The responses to these antigens were enhanced markedly by LPS in whole and macrophage‐depleted spleen cells. The enhancement was greater in the latter group than in the former. The adjuvant effect among whole, T cell‐depleted, macrophage‐depleted and both macrophage‐ and T cell‐depleted spleen cells was compared. The response to TNP‐Ficoll was enhanced markedly by LPS in all groups. The enhancement was greater in the latter two groups than in the first two groups. The response to TNP‐KLH was enhanced by LPS strongly in macrophage‐depleted spleen cells, moderately in whole and both macrophage‐ and T cell‐depleted spleen cells, and only slightly in T cell‐depleted spleen cells. Enhancement was restored to T cell‐depleted spleen cells by adding T cells. The response to TNP‐KLH of macrophage‐depleted spleen cells of LPS‐responsive C3H/HeN mice which was enhanced by LPS was suppressed by adding splenic macrophages of C3H/HeN mice, but not of LPS‐nonresponsive C3H/HeJ mice. The response to TNP‐KLH of macrophage‐depleted spleen cells of C3H/HeJ mice was not enhanced by LPS, irrespective of the addition of macrophages of C3H/HeN mice. The results indicate that B cells are activated directly by LPS, and T cells enhance and macrophages suppress the adjuvant effect of LPS.