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Purine Metabolic Enzymes in Lymphocytes: I. Adenosine Deaminase and Purine Nucleoside Phosphorylase Activities in Mouse Lymphocyte Subpopulations
Author(s) -
Kurashige Satonori,
Akuzawa Yuki,
Yoshida Toshiharu,
Teshima Chisato,
Mitsuhashi Susumu
Publication year - 1982
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1982.tb00155.x
Subject(s) - adenosine deaminase , purine nucleoside phosphorylase , biology , spleen , concanavalin a , lymphocyte , purine , microbiology and biotechnology , 5' nucleotidase , adenosine , endocrinology , medicine , enzyme , in vitro , biochemistry , immunology
The distribution of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities in lymphoid organs and lymphocyte subpopulations in mice, and the effect of phytohemagglutinin P (PHA‐P) and concanavalin A (Con A) on the enzyme activities were studied. ADA activity was distributed equally in cells from all organs used and no mouse strain differences were observed. In contrast, PNP activity varied with the mouse strain, being highest in C57BL/6 mice and lowest in BALB/c mice, and with the organ in ICR mice, being high in peripheral blood lymphocytes and spleen lymphocytes, low in mesenteric lymph node cells and absent or very weak in thymus cells. T and B lymphocytes were prepared from spleens of ICR mice. High ADA activity was found in both T and B lymphocytes, whereas PNP activity in the T lymphocytes was about one‐third of that in the B lymphocytes. PNP activity in thymus cells was increased to the normal level of T lymphocytes in the spleens by cultivation without stimulant. The development of PNP activity in thymus cells was partially inhibited by Con A but was not affected by PHA‐P. ADA activity in thymus cells was enhanced by in vitro stimulation with PHA‐P but not with Con A. In contrast, in spleen lymphocytes the development of ADA activity was enhanced by stimulation with PHA‐P and Con A, and that of PNP activity was enhanced by PHA‐P but not by Con A.