Premium
A Simplified Micro‐Method for Cytotoxicity Testing Using a Flat‐Type Titration Plate for the Detection of H‐2 Antigens
Author(s) -
Shiroishi Toshihiko,
Sagai Tomoko,
Moriwaki Kazuo
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00141.x
Subject(s) - glutaraldehyde , titration , cytotoxicity , reproducibility , chromatography , staining , biology , biomedical engineering , materials science , chemistry , in vitro , biochemistry , medicine , inorganic chemistry , genetics
A new micro‐method for cytotoxicity testing using a flat‐type titration plate has been developed. This micro‐method is so simple that it involves no washing and staining procedures. In this method, cell viability is judged on the basis of the refractility change in the lymphocytes seen under the phase‐contrast microscope. This micro‐method has higher sensitivity and reproducibility than the dye exclusion method and 51 Cr‐release assay. Fixation of cells with glutaraldehyde and tight covering of the plate allow us to read the result even after 3 weeks. These advantages of the new method enable us to deal with a large number of samples at one time. We also introduce a rapid technique for the preparation of a cell suspension from the lymph node without killing the animal.