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Suppression of Hen Egg‐White Lysozyme (HEL)‐Specific Delayed‐Type Hypersensitivity Responses in Mice by Suppressor T Cells after Neonatal Administration of Anti‐Idiotypic Antibodies
Author(s) -
Semma Masanori,
Amano Tsunehisa,
Fujio Hajime,
Sakato Nobuo
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00139.x
Subject(s) - biology , lysozyme , antiserum , spleen , antibody , immunology , antigen , suppressor , in vitro , antibody response , delayed hypersensitivity , c57bl/6 , microbiology and biotechnology , gene , biochemistry , genetics
Anti‐idiotypic rabbit antiserum (anti‐Id) directed to the idiotypes of anti‐hen egg‐white lysozyme (HEL) antibody from a single C3H mouse (No. 2) was shown to be capable of recognizing only a fraction of the anti‐HEL antibody populations produced by other C3H mice. Experiments were performed to examine the effect of this particular anti‐Id on the delayed‐type hypersensitivity (DTH) response specific for the same protein antigen. A group of 60‐day‐old C3H mice which had been administered anti‐Id within 24 hr after birth were tested for HEL‐DTH response. The results indicated that the DTH response was completely suppressed by the anti‐Id treatment. The inhibition of DTH reactivity is due to active suppression and involves the generation of suppressor T cells. Thus, the suppression induced with a single injection of anti‐Id was transferable with both spleen cells and thymocytes from mice that received anti‐Id. These suppressor cells are T cells since their ability to suppress DTH is completely abrogated by treatment in vitro with anti‐Thy 1.2 serum and complement.