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Analysis of the Inhibitory Effect of Canavanine on the Replication of Influenza RI/5 + Virus: II. Interaction of M Protein with the Plasma Membrane
Author(s) -
Aoki Hiizu,
Maeno Koichiro,
Tsurumi Tatsuya,
Takeura Shigeki,
Shibata Motohiro,
Hamaguchi Michinari,
Nagai Yoshiyuki,
Sugiura Yasuo
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00137.x
Subject(s) - canavanine , biology , hemagglutinin (influenza) , virus , orthomyxoviridae , nucleoprotein , microbiology and biotechnology , arginine , biochemistry , membrane , influenza a virus , virology , amino acid
When influenza A/RI/5 + virus‐infected cells were incubated in medium to which 2 μ g of canavanine (arginine analog) per ml had been added 4 hr after infection, all viral polypeptides were synthesized but the budding‐like process with the appearance of extracellular virus was completely inhibited. The plasma membrane isolated from these cells contained exclusively hemagglutinin (HA), and membrane (M) protein and nucleoprotein (NP) appeared to be associated with the nucleus, in contrast to untreated cells whose plasma membrane contained abundant HA, M protein, and NP. Disruption of canavanine‐treated cells by freeze‐thawing generated a number of hemagglutinating membranous vesicles or fragments containing exclusively HA. By isotope labeling it was found that the M protein synthesized in the presence of canavanine, together with HA and NP, is a canavanine‐substituted polypeptide. It is suggested that canavanine inhibits the formation of the mature envelope of influenza RI/5 + , because of the inability of M protein to associate with the plasma membrane.