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Expression of Herpes Simplex Virus Type‐Common Surface Antigens in Clonal Cells of an HSV Type 2‐Transformed Line: Effect of Adriamycin
Author(s) -
Kako Daijiro,
Ohnishi Yoshinari,
Kimura Susumu
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00136.x
Subject(s) - antipain , biology , herpes simplex virus , clone (java method) , puromycin , microbiology and biotechnology , cell culture , dna synthesis , antigen , colchicine , virology , virus , protease , protein biosynthesis , dna , biochemistry , enzyme , leupeptin , immunology , genetics
The expression of herpes simplex virus (HSV) type‐common surface antigens (CSA) in a representative cell clone (155–4‐03) of hamster cell line 155–4 transformed by HSV type 2 was enhanced by treatment with inhibitors of RNA synthesis [adriamycin (ADM) and daunomycin] but not with inhibitors of DNA synthesis (2‐iododeoxyuridine, bleomycin, mitomycin C and cytosine arabinoside), although all these drugs decreased the number of viable cells to a similar extent. ADM‐enhanced CSA expression in the clone was inhibited by puromycin and 2‐deoxy‐D‐glucose, suggesting that the enhanced expression required both protein synthesis and glycosylation. This enhanced expression was sensitive to protease inhibitors (antipain and p ‐nitrophenyl‐ p '‐guanidinobenzoate) and procaine, which is known to inhibit trypsin action and the organization of cell membrane‐associated cytoskeletal elements (microfilaments and microtubules). Furthermore, low concentrations of ADM (0.1 μ g/ml) and actinomycin D (0.5 μ g/ml) enhanced CSA expression additively, but the most effective concentrations of ADM (0.25 μ g/ml) and actinomycin D (2 μ g/ml) did not. These findings indicate that the two drugs enhance CSA expression in the clone by a common mechanism.