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Biological and Biochemical Characterization of Macrophage Activating Factor (MAF) in Murine Lymphocytes: Role of Mannopyranosyl Residue of the MAF Molecule in Macrophage Activation
Author(s) -
Fukazawa Yoshimura,
Kagaya Keiko,
Miura Hiroshi
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00124.x
Subject(s) - macrophage activating factor , intracellular , concanavalin a , macrophage , biology , mannose , sepharose , lectin , sephadex , biochemistry , microbiology and biotechnology , in vitro , enzyme
Activation of macrophages with macrophage activating factor (MAF) was evaluated by measuring the intracellular killing activity of murine macrophages against Salmonella typhimurium . Concanavalin A (Con A)‐induced MAF‐rich fraction was obtained by a Sephadex G‐100 column, which contained molecules ranging from 25,000 to 67,000 daltons. The intracellular killing ability of mouse peritoneal macrophages against S. typhimurium was found to be increased by 0.1 m d‐mannose as well as by Con A‐induced MAF‐rich fraction. Both 0.1 m d‐mannose and MAF exhibited a similar timing pattern for macrophage activation. The same concentration of d‐glucose or l‐rhamnose did not change bacterial uptake and intracellular killing by macrophages. Moreover, when MAF‐rich fraction was applied to a Con A‐Sepharose column, a fraction that was adsorbed on Con A and eluted with 0.1 m α ‐methyl d‐mannoside exhibited MAF activity. These results suggest the possibility that mannopyranosyl residues in the MAF molecules play an important role as a ligand in macrophage activation.