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Comparison of Three Electron Microscopy Techniques for the Detection of Human Rotaviruses
Author(s) -
Trépanier Pierre,
Alain Robert,
Micusan Valeriu,
McLaughlin Bernadette,
Berthiaume Laurent
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00108.x
Subject(s) - electron microscope , rotavirus , microscopy , biology , virus , immune system , virology , physics , optics , immunology
Rotavirus detection by direct electron microscopy was compared with direct and indirect immune electron microscopy techniques. The latter two approaches permitted the enumeration of 25 and 103 times more rotaviruses respectively, than direct electron microscopy. Also, 70% and 90% of the virus particles were aggregated by direct and indirect immune electron microscopy techniques respectively, thus facilitating their detection.