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Enhancement of Cytotoxicity of Human Peripheral Blood Lymphocytes by Interferon
Author(s) -
Kato Tsuneo,
Minagawa Tomonori
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00087.x
Subject(s) - cytotoxicity , biology , cytotoxic t cell , peripheral blood mononuclear cell , virology , antibody , interferon , mumps virus , natural killer cell , immunology , antigen , alpha interferon , microbiology and biotechnology , interferon gamma , cytokine , in vitro , biochemistry
Human lung carcinoma cells persistently infected with mumps virus (Pc‐10/MpV) were lysed with human peripheral blood mononuclear leukocytes (PBML) obtained from seropositive donors who had anti‐mumps virus‐neutralizing antibody in their sera. This cellular cytotoxicity was due not to the cytotoxic T lymphocytes but mainly to the non‐T, non‐B cells, possibly related to natural killer (NK) cells. Moreover, it was concerned not with antibody against mumps virus antigens but with alpha‐interferon (IFN‐ α ) produced in the mixture of human PBML and Pc‐10/MpV cells, since this cellular cytotoxicity was suppressed by anti‐human IFN‐ α rabbit serum. Exogeneous IFN‐ α augmented the cytotoxicity of non‐T, non‐B cells, not T cells, for the uninfected Pc‐10 cells. IFN‐ γ that had been induced by heat‐killed Listeria monocytogenes in PBML had the same capacity to augment NK activity did IFN‐ α .