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Phenotypic Change of an SV40‐Transformed Mouse Macrophage Line, BB‐W‐531–2, Induced by Different Cultural Methods
Author(s) -
Takayama Hisao,
Tanigawa Takahiko,
Takagi Atsushi
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00086.x
Subject(s) - macrophage , clone (java method) , biology , phagocytosis , cell culture , microbiology and biotechnology , phenotype , immune system , immunology , in vitro , genetics , dna , gene
A simian virus 40 (SV40)‐transformed macrophage clone which was established from BALB/cAnN mouse bone marrow cells was used to study the effect of different cultural conditions on the expression of macrophage properties. The macrophage clone, BB‐W‐531–2 line, expressed and maintained the macrophage properties, immune phagocytosis or Fc‐ and complement receptors, under the growth‐inhibiting conditions of confluent density and of cultivation on bacteriologic dishes with reduced adhesiveness. However, the cells lost their ability to express the macrophage properties dependent upon cell density after repeated culture splits in the growing phase. These cells regained that ability when they were cocultured with cells having macrophage properties. These results suggest that there is a possible correlation between reduced multiplication and the expression of macrophage properties, and that macrophage properties which have been suppressed or blocked may be induced by diffusible factor(s) produced by macrophages.