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Analysis of Human T‐Cell Antigens by One‐ and Two‐Dimesional Polyacrylamide Gel Electrophoresis
Author(s) -
Fujimoto Junichiro,
Ishii Yoshifumi,
Kon Shinichiro,
Matsuura Akihiro,
Kikuchi Kokichi
Publication year - 1981
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1981.tb00074.x
Subject(s) - biology , polyacrylamide gel electrophoresis , antigen , gel electrophoresis , computational biology , electrophoresis , microbiology and biotechnology , genetics , biochemistry , enzyme
One‐ and two‐dimensional polyacrylamide gel electrophoresis (PAGE) was performed on immunoprecipitates formed between anti‐human T‐cell xenoantiserum (ATS) and cell‐surface glycoproteins of human lymphocytes, that had been radioiodinated by lactoperoxidase and purified on a Lentil lectin‐coupled Sepharose 4B column. In some experiments, the cells were 3 H‐labeled by periodate‐tritiated borohydride. ATS that was absorbed with B cells recognized a number of cell‐surface antigens expressed preferentially on human thymus and T cells, with molecular weights of 150K (T150), 94K (T94), 72K (T72), and 65K (T65) daltons. Whereas T150 appeared to consist of multiple components of heavily sialylated glycoproteins and to be expressed largely on thymus and T cells, and to a much lesser extent on B cells, the remaining T94, T72, and T65 glycoproteins seemed to be present on thymus and T cells but absent from B cells. Two‐dimentional PAGE analysis of these T‐cell glycoproteins precipitated by ATS demonstrated that T94 was an acidic glycoprotein with p I of 4, while T72 and T65, the latter being found on thymus and T cells but not on T cell‐type leukemic cells, exhibited marked electric charge heterogeneity with p I ranging from 4 to 7. These data clearly suggest that human thymus and T cells possess a complex antigenic make‐up on their cell surfaces, comparable to that of mouse T cells with a variety of Ly antigen systems.